Plant or Fungal Cell Dissociation Technique?
Posted 02 July 2010 - 08:31 AM
Does anyone know of any other techniques used to dissociate clumps of plant or fungal cells? Thanks!
Posted 04 July 2010 - 11:19 PM
other techniques widely used for fungi (mainly moulds) are 0.01% Tween 80 solution, adding a drop of 70% EtOH to your microscopy slide or sonication.
Let me know what worked, as Bd seems to be invading Austria as well now;
Edited by gebirgsziege, 04 July 2010 - 11:24 PM.
Posted 06 July 2010 - 07:46 AM
I tried resuspending the colonies in a 1:1 mixture with nutrient broth and 70% ethanol, then in 70% ethanol with no nutrient broth. Neither disrupted the colonies. I haven't tried your other suggestions yet.
Posted 06 July 2010 - 10:37 PM
Tween or other mild detergents often helps a lot; just trie to mix your culture with it and shake for some time (half an hour) on a head over head shaker to dispens your cells