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luciferase


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#1 soymilk14

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Posted 02 July 2010 - 04:58 AM

i want to ask about luciferase assay. can you share some complete site for luciferase assay starting with constructing and the appropriate controls?

Can i transfect luciferase contruct with my promoter of interest and co-transfect sirna to the cell too to knockdown a transcription factor that i suspect controls the promoter of my gene of interest? thank you

#2 epibio

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Posted 02 July 2010 - 06:57 AM

Try Promega's excellent guide on luciferase reporter systems:

http://www.promega.c...guide/chap8.htm




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