Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

protein overexpression


  • Please log in to reply
3 replies to this topic

#1 Puteri Q

Puteri Q

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 01 July 2010 - 07:26 PM

Hi there...
Ive been doing my research about protein overexpression. Is there anyone who knows any factor that may affect to protein expression?
Does the enviromental conditon (ex. medium) may affect to the solubility of the recombinant protein?

P.S.: transforman==> E.coli BL21 DE3 Plyss

--Q--
:lol:

#2 perneseblue

perneseblue

    Unlimited ligation works!

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 578 posts
17
Good

Posted 05 July 2010 - 01:22 PM

Hi there...
Ive been doing my research about protein overexpression. Is there anyone who knows any factor that may affect to protein expression?
Does the enviromental conditon (ex. medium) may affect to the solubility of the recombinant protein?

P.S.: transforman==> E.coli BL21 DE3 Plyss

--Q--
:P


Yes, the environment does affect the solubility and expression of recombinant protein. This is why we spend so much money and time optimising growth condition. And it is also the reason why it is so difficult to scale large bio reactors.

Examples of factors that affect protein expression,
Media Carbon-nitrogen ratio.
Carbon source composition.
Nitrogen source composition/availability.
Temperature
Oxygen availability
Waste product build up
Cellular growth rate
pH

It is complicated and i only touched the periphery of this field when I was reading up factors that influence plasmid production and cell growth density.
May your PCR products be long, your protocols short and your boss on holiday

#3 Puteri Q

Puteri Q

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 08 July 2010 - 09:33 PM

Hi there...
Ive been doing my research about protein overexpression. Is there anyone who knows any factor that may affect to protein expression?
Does the enviromental conditon (ex. medium) may affect to the solubility of the recombinant protein?

P.S.: transforman==> E.coli BL21 DE3 Plyss

--Q--
B)


Yes, the environment does affect the solubility and expression of recombinant protein. This is why we spend so much money and time optimising growth condition. And it is also the reason why it is so difficult to scale large bio reactors.

Examples of factors that affect protein expression,
Media Carbon-nitrogen ratio.
Carbon source composition.
Nitrogen source composition/availability.
Temperature
Oxygen availability
Waste product build up
Cellular growth rate
pH

It is complicated and i only touched the periphery of this field when I was reading up factors that influence plasmid production and cell growth density.



Ooo..thanks for the answer. :) Do you have any suggestion for the reference that I could learn about those factors?

#4 perneseblue

perneseblue

    Unlimited ligation works!

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 578 posts
17
Good

Posted 09 July 2010 - 07:49 PM

I looked up pubmed and google.

Just start off with a simple inquiry like "protein expression improvement enhancements". That should get you a few papers and reviews. Read them up and you'll pick up more specific terminology and become familiar with the authors who work in this field. Then you can use the correct terminology to improve your search.

And also look up the US patent office. You will probably find most of the more advance/recent work in protein expression enhancements "missing". But by looking at the patent office, you can get a glimpse of what modern companies are doing now. Protein expression is big money.
May your PCR products be long, your protocols short and your boss on holiday




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.