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NUCLEAR PROTEIN PREPARATION


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#1 nanaz

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Posted 01 July 2010 - 06:17 PM

Hi
I am looking for a specific transcription factor by western and it seems to come up only frequently, can you tell me the exact way i should extract nuclear proteins and prepare them for western from cultures cells and also if you know from tissues? thanks

#2 lsek

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Posted 01 July 2010 - 07:40 PM

Hi,

I don't have a specific protocol to share. However, you could search in google this term "cellular fractionation protocol cell and tissue".

Bests

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