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some questions

Started by noyara, Jul 01 2010 09:58 AM

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7 replies to this topic

#1 noyara

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Posted 01 July 2010 - 09:58 AM

Hi all,

Is there any website can give me the amino acid sequence and DNA sequence for specific protein ...?
and also I have another question, do u know any successful protocol used for mRNA isolation from corn leaves...?
many thanks in advance..

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#2 hobglobin

Growing old is mandatory, growing up is optional...

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Posted 01 July 2010 - 10:11 AM

noyara, on Jul 1 2010, 07:58 PM, said:

Hi all,

Is there any website can give me the amino acid sequence and DNA sequence for specific protein ...?

many thanks in advance..

Try NCBI...: http://www.ncbi.nlm.nih.gov/

One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

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#3 noyara

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Posted 01 July 2010 - 01:41 PM

hobglobin, on Jul 1 2010, 10:11 AM, said:

noyara, on Jul 1 2010, 07:58 PM, said:

Hi all,

Is there any website can give me the amino acid sequence and DNA sequence for specific protein ...?

many thanks in advance..
:lol:

Try NCBI...: http://www.ncbi.nlm.nih.gov/

Many Thanks for your help..

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#4 muna ismail

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Posted 03 July 2010 - 06:49 AM

I have a question which I suffer from the degrdation of blood when extracted serum from the blood of mice infected with
During the withdrawal of blood from the mice eyes, it affects my results
I hope to provide me with any information or sites containing information that will help me to prevent decomposition
Please note that I leave the blood of 37 degrees Celsius for half an hour

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#5 muna ismail

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Posted 03 July 2010 - 07:39 AM

i have problem in getting clear serum from mice blood. i collect the blood from orbital sinus (eye), keep it at 37 C for 1 hr. then keep it in fridge (4 degree C) for 3-4 hrs. but when i centrifuge it, blood gets hemolysed. this prob doesn't occur with rabbit blood. whats the reason for the hemolysis, because i handle the sample very gently? please help..

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#6 noyara

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Posted 04 July 2010 - 01:15 AM

muna ismail, on Jul 3 2010, 07:39 AM, said:

i have problem in getting clear serum from mice blood. i collect the blood from orbital sinus (eye), keep it at 37 C for 1 hr. then keep it in fridge (4 degree C) for 3-4 hrs. but when i centrifuge it, blood gets hemolysed. this prob doesn't occur with rabbit blood. whats the reason for the hemolysis, because i handle the sample very gently? please help..

Dear Muna,
I have no idea about ur problem... u can post ur question in the main page to read by other staff...
regards