7 replies to this topic

[moljul]

how can i bring cells onto a slide without cytocentrifugation?

thanks for recommendation

[laurequillo]

moljul, on Jul 1 2010, 01:22 PM, said:

how can i bring cells onto a slide without cytocentrifugation?

thanks for recommendation

you can seed them on the top of a cover glass ( I am not sure if that is your question...).

Edited by laurequillo, 01 July 2010 - 03:41 AM.

[moljul]

laurequillo, on Jul 1 2010, 04:40 AM, said:

moljul, on Jul 1 2010, 01:22 PM, said:

how can i bring cells onto a slide without cytocentrifugation?

thanks for recommendation

you can seed them on the top of a cover glass ( I am not sure if that is your question...).

not really feasible. i want to observe my macs sorted cells under the microscope, therefore i have to bring them onto a slide!

[SuMi]

If you just want a quick look at your cells you can just drop 10 µl onto a glass slide and put a coverslip over the drop. Then you can view them under the microscope

Edited by SuMi, 01 July 2010 - 09:10 AM.

[moljul]

SuMi, on Jul 1 2010, 10:09 AM, said:

If you just want a quick look at your cells you can just drop 10 µl onto a glass slide and put a coverslip over the drop. Then you can view them under the microscope

in that case they have to be as concentrated as possible??

[SuMi]

No not at all. I did it last week with 10 µl used directly after separation which included a wash through with 500 µl buffer so they were quite dilute

[Inmost sun]

moljul, on Jul 1 2010, 11:22 AM, said:

how can i bring cells onto a slide without cytocentrifugation?

thanks for recommendation

as suggested, seeding on cover glas is a good idea; round coverglas is to prefer for round wells of cell culture plates; if cells do not grow on glas, try coating with collagen, fibronectin, matrigel etc

[laurequillo]

Inmost sun, on Jul 2 2010, 09:13 PM, said:

moljul, on Jul 1 2010, 11:22 AM, said:

how can i bring cells onto a slide without cytocentrifugation?

thanks for recommendation

as suggested, seeding on cover glas is a good idea; round coverglas is to prefer for round wells of cell culture plates; if cells do not grow on glas, try coating with collagen, fibronectin, matrigel etc

Or poly-l-lysine. I did not get why you can not seed them. But anyway, of course you can just take a look directly from the flask, just puting some of them in a cover. But if you dont need to stain them or anything you can just check from the plate or the flask in the microscope. You just put the plate there and check whatever you are interested in (gfp or morphology or whatever...)