Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

need lysis buffer for histone extraction


  • Please log in to reply
1 reply to this topic

#1 deep

deep

    member

  • Active Members
  • Pip
  • 18 posts
0
Neutral

Posted 30 June 2010 - 04:37 PM

Hello all,
Can anyone tell me how to extract histones from cells for western blot? or is their any lysis buffer especially used for histones extraction?
thanking you..

#2 laurequillo

laurequillo

    The Goddamn Batman!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 265 posts
1
Neutral

Posted 30 June 2010 - 07:42 PM

Hello all,
Can anyone tell me how to extract histones from cells for western blot? or is their any lysis buffer especially used for histones extraction?
thanking you..


I ued some antiboides against H3 and some histone modifications, and the lysis buffer I used was quite standard. You can use NP-40 I guess or do the lysis directly in sds 1x
"He must be very ignorant for he answers every question he is asked" Voltaire

"This is SPARTA!"

"Im the goddamn batman"




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.