Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Storage of Fixed Cells for Immunofluorescence staining


  • Please log in to reply
1 reply to this topic

#1 Merrique

Merrique

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 30 June 2010 - 04:31 AM

Dear all,
I want to do a time course experiment with a tumor cell line over 10 days. I have to induce every plate of cells at one day and then harvest every day over ten days. I cannot do it vice versa. This is a problem, as I will simultaneously need to make samples for IF (on coverslips in the 10 cm plates). This would mean I have to do staining every day, which is 4 hours for 4 slides or so... I would prefer to stain on one day and store the coverslips somehow until then. Also, I would have the same staining conditions, then.
Do you know a method to keep fixed cover slips for several days until staining?

#2 rkay447

rkay447

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 180 posts
20
Excellent

Posted 02 July 2010 - 03:27 PM

Dear all,
I want to do a time course experiment with a tumor cell line over 10 days. I have to induce every plate of cells at one day and then harvest every day over ten days. I cannot do it vice versa. This is a problem, as I will simultaneously need to make samples for IF (on coverslips in the 10 cm plates). This would mean I have to do staining every day, which is 4 hours for 4 slides or so... I would prefer to stain on one day and store the coverslips somehow until then. Also, I would have the same staining conditions, then.
Do you know a method to keep fixed cover slips for several days until staining?

Just store them at 4 degree in PBS. I do this all the time and you can hold coverslips for weeks.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.