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DNA extraction form endoscopy samples


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4 replies to this topic

#1 queen

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Posted 30 June 2010 - 03:53 AM

hello

i am doing research about helicobacter pylori and my problem is i am not sure that i can exract dna from biopsy samples by single dna extraction using CTAB/NACL and chlorophrom method
i dont want to cultre it
please help me :D

#2 Curtis

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Posted 01 July 2010 - 12:07 AM

you just need the DNA to run PCR, right? so you wouldn't need to extract from all the sample. just a small piece would give you enough DNA to run PCR.

hello

i am doing research about helicobacter pylori and my problem is i am not sure that i can exract dna from biopsy samples by single dna extraction using CTAB/NACL and chlorophrom method
i dont want to cultre it
please help me :D



#3 queen

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Posted 04 July 2010 - 01:12 AM

hello
i didnt get you sorry
i know it bult my problem is about the method which i use i doubt about it and i didnt get any result form PCr for UreaC ;)

#4 epibio

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Posted 06 July 2010 - 06:37 AM

If you're using the DNA just for PCR, try the QuickExtract solution from Epicentre. It works well with a variety of tissue samples.

#5 an15

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Posted 13 March 2011 - 11:35 PM

hi

I want to extract Helicobacter pylori`s DNA from biopsies (i got mine frozen in -80c). i was told to avoid phenol/chloroform method, but i cant find any method that not using Phenol/chloroform in extracting bacteria DNA. If i use any commercial kits for extracting DNA, will i get a pool of bacteria/human DNA and later use PCR to find Helicobacter?

Please help. Thanks )




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