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Assay Collagen Crosslink


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#1 JHu

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Posted 29 June 2010 - 12:24 PM

Hi, I am trying to measure the degree of collagen crosslink in a 3 D tissue construct we created in the lab. I have done some research, and many of the publication pointed to the uses of radioactive-labeled proline. However, our lab does not have a license on using any radioactive materials. Does anyone have recommendation on assays we can run to determine the collagen crosslink or know any place we can send the samples to for measurement?

Thank you,
JHu

#2 Maddie

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Posted 30 June 2010 - 06:29 AM

What about a comet assay?
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

A. Einstein

#3 JHu

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Posted 30 June 2010 - 06:51 AM

What about a comet assay?


Isn't comet assay for DNA damage determination ?

#4 Maddie

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Posted 30 June 2010 - 10:59 AM

Yes it is, but I think you can also use it to measure crosslinks.
Check this article
http://clincancerres...nt/5/3/507.full

(PS I should mention that I never used the assay myself though :lol: )
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

A. Einstein




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