Its a non-reduced sample...
can u suggest reasons as to why this might happen??!!! is the salt concentration (1M NaCl) of the sample playing any role here??!!! i get these kind of profile in 140 mM salt also!!!
I run at 130V constant for one hour!!
Its a Lamellei buffer system!!
any furtehr details i can provide if u need!!!
Edited by Prep!, 29 June 2010 - 12:40 AM.