I use invitrogen one shot high effeciency chemically competant cell. I have even checked the transformation effeciency by positive control with the uncut vector and got good no. of colonies. so I am sure the problem is in ligation. I have tried both invitrogen and fisher sci. T4 DNA ligase enzymes.
Please suggest me anything else I can try.
Thankyou in advance
Edited by pragathi, 28 June 2010 - 06:52 AM.