problem about DNA electrophoresis
Posted 28 June 2010 - 01:45 AM
Posted 28 June 2010 - 04:59 AM
Dear everyone, I have a problem about the DNA electrophoresis. when I use the big well to run the DNA electrophoresis(in order to extraction the pcr product from gel), the sample can not settle down completely. The DNAs become two layers and there are no DNAs in the middle of the gel. When I use the small well, everything is ok. I do not know what happened, could you give me some suggestions! Thanks very much!
Posted 28 June 2010 - 05:31 AM
If the former, then you have two different DNA molecules present in your sample which have been separated by size through electrophoresis. If the latter, then it's likely the stain didn't penetrate the gel completely, and this staining artifact is of no consequence, but likely could be overcome by staining the gel longer to allow complete penetration of the gel.
Posted 28 June 2010 - 08:08 AM
Posted 30 June 2010 - 12:21 AM