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how to find TSS of a certain gene


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#1 lacee.s

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Posted 27 June 2010 - 06:35 PM

Hello everyone, I am now trying to predict the promoter region and TSS of a certain gene on chicken for methylation study. But I have no idea to achieve that. I saw some online program can be used to find those regions on human genes but not for chicken input. Can anybody give me some suggestions. Thanks a lot!

#2 pcrman

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Posted 27 June 2010 - 11:20 PM

The resources available for determining TSS is certainly much less for chicken genes than human and mouse genes. You can just find the start of the first exon (or transcription start), the promoter sequence is usually immediately upstream of the TSS. If there is CpG islands in the vicinity, it is pretty much sure that is the promoter sequence.




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