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Annexin and propidium staining


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#1 shldbcrzy

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Posted 25 June 2010 - 12:57 PM

Does any one work with these apoptosis markers in lymphoblasts, i just need to knw the procedure

thanks in advance!

#2 Rsm

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Posted 28 June 2010 - 12:38 AM

Follow the manufacturer's recommendation for Annexin V (use the right buffer etc), and add PI to a final conc of 50ng/ml. Use unstained - only AnnV - only PI as negative control and for compensation.

Cheers,

Minna
I got soul, but I'm not a soldier




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