Jump to content


- - - - -

Annexin and propidium staining


1 reply to this topic

#1 shldbcrzy

    Enthusiast

  • Active Members
  • PipPip
  • 25 posts

Posted 25 June 2010 - 12:57 PM

Does any one work with these apoptosis markers in lymphoblasts, i just need to knw the procedure

thanks in advance!

#2 Rsm

    Post Dog

  • Active Members
  • PipPipPipPipPip
  • 357 posts

Posted 28 June 2010 - 12:38 AM

Follow the manufacturer's recommendation for Annexin V (use the right buffer etc), and add PI to a final conc of 50ng/ml. Use unstained - only AnnV - only PI as negative control and for compensation.

Cheers,

Minna
I got soul, but I'm not a soldier





Home - About - Terms of Service - Privacy - Contact Us

©1999-2011 Protocol Online, All rights reserved.