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are vacuum manifolds (plasmid prep) interchange with brands?

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#1 blue800



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Posted 24 June 2010 - 07:55 PM

Hey all,
I'm wondering if anyone has experience with vacuum manifolds for high throughput plasmid preps. I need to do a megaprep using a Genelute (sigma) kit...it seems that most megaprep kits need a vacuum manifold, but I only have access to a biorad version.

My question is: Are the kits/manifolds interchangable with brands or do the different companies modify them to only be used with their own products?

Thanks in advance,
Eric J.

#2 swanny



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Posted 24 June 2010 - 09:01 PM

As long as you get a good seal to allow the liquid to be pulled through, and as long as you don't get cross-contamination, you should be fine. The most important issue is getting a seal; if that doesn't work, you're plumb out of luck!
Heart disease kills more women than breast cancer, but heart attack symptoms differ from men's symptoms. Get to know your heart... it could save your life.

#3 bob1


    Thelymitra pulchella

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Posted 27 June 2010 - 04:12 PM

Most vacuum manifolds use a Luer system which is the common tip for all syringes. I have used invitrogen, promega and a third brand (which I forget now) on a manifold from promega.

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