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Phospholylation of primers


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#1 snpsnooper

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Posted 24 June 2010 - 01:07 PM

I forgot to PNK treat my primers before PCR, just wondering if I should not use this for cloning into my CIP treated vector. Thanks for any feedback.

#2 epibio

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Posted 25 June 2010 - 12:20 PM

You could still phosphorylate after isolating your PCR product.




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