I have cloned a gene into a reporter plasmid (EGFP-tagged) and the band I am seeing is 10kDa heavier than what I expected. The band is around 70kDa, but I was expecting 60kDa by my calculations.
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Does the tagged-protein always appear at the predicted size?
Started by Curtis, Jun 23 2010 08:20 PM
1 reply to this topic
#1
Curtis
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Posted 23 June 2010 - 08:20 PM
#2
laurequillo
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Posted 23 June 2010 - 08:31 PM
Curtis, on Jun 24 2010, 06:20 AM, said:
I have cloned a gene into a reporter plasmid (EGFP-tagged) and the band I am seeing is 10kDa heavier than what I expected. The band is around 70kDa, but I was expecting 60kDa by my calculations.
Yeah, It is not a problem. Somtimes the run differently when they have a tag. And GFP is quite big, so It affects big time your protein, and it is not unusual to see a different size than expected
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