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Does the tagged-protein always appear at the predicted size?


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#1 Curtis

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Posted 23 June 2010 - 08:20 PM

I have cloned a gene into a reporter plasmid (EGFP-tagged) and the band I am seeing is 10kDa heavier than what I expected. The band is around 70kDa, but I was expecting 60kDa by my calculations.

#2 laurequillo

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Posted 23 June 2010 - 08:31 PM

I have cloned a gene into a reporter plasmid (EGFP-tagged) and the band I am seeing is 10kDa heavier than what I expected. The band is around 70kDa, but I was expecting 60kDa by my calculations.


Yeah, It is not a problem. Somtimes the run differently when they have a tag. And GFP is quite big, so It affects big time your protein, and it is not unusual to see a different size than expected
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