need colony formation assay protocol
Posted 22 June 2010 - 05:58 PM
Can anyone please send me protocol for colony formation assay ( not soft agar gel colony assay).
in this method colonies are stained with crystal violet or methylene blue after seedind in 6 well plate.
Posted 22 June 2010 - 10:16 PM
Posted 28 June 2010 - 08:18 PM
thank you for help.
what I did is I seeded cells 1000 per well
next day added drug
after 48 hrs removed drug
Now I will do procedure as told by you. afer that how can I analyse my data? is there any formula to calculat percentage of colony formed in drug treated cells as compared to control?
i will appreciate your help.
Edited by deep, 28 June 2010 - 08:57 PM.
Posted 28 June 2010 - 11:32 PM