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QPCR/DDCT Method/ Statistics


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#1 George250

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Posted 22 June 2010 - 05:49 PM

Hi

I need your help.. I did a QPCR using the DDCt method and I would like to compare the 5 RQ values (actually i would like to compare 5 numbers) that I got. How can I compare the RQ values for statistical significance? I did not repeat the experiment so I can get means.

thanx

#2 tea-test

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Posted 23 June 2010 - 04:09 AM

hi, what are these 5 samples? different treatments? i would say you need biological replicates within your different treatment groups (at least 3) to find out first the variance within one treatment group in order to compare different groups.
tea-test: The artist formerly known as Ned Land

#3 George250

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Posted 23 June 2010 - 04:47 AM

hi, what are these 5 samples? different treatments? i would say you need biological replicates within your different treatment groups (at least 3) to find out first the variance within one treatment group in order to compare different groups.


hi, thanx for your answer.. yes, my samples are tissues treated differently. i do have e biological replicates within my groups.




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