Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

LINE1 OFR2 Primer Design

  • Please log in to reply
1 reply to this topic

#1 Michelle0505



  • Members
  • Pip
  • 4 posts

Posted 22 June 2010 - 02:57 PM

Hey Everyone,

I'm trying to design a primer that will detect ORF2 in LINE1 in humans. I know that basically what I"m supposed to do is find the sequence, and design a primer complementary to it, but I have no idea where or how to start. Any help would be much appreciated!

Thank you all in advance ;)

#2 Trof


    Brain on a stick

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,357 posts

Posted 23 June 2010 - 05:10 AM

What do you want the primers for exactly?

There is no easy way to design primers for transposons. As you know there are placed all over human genome, so any primer specific for L1 sequence will detect all of them. If you want to detect a transposon in one specific location (which I assume is what you want) you must design primers partialy for the L1 sequence any partialy for the gene where it's inserted (so you must know the exact site of insertion). You can search NCBI Nucleotide database for L1 retroelement in your specific sequence, if it was published. Also there is L1base with sequences of L1 elements.

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.