rna isolation manually
Posted 22 June 2010 - 09:15 AM
I am doing rna isolation manually by the phenol/sds method from plant source . After running the rna on agarose denaturing gel i got 2 intact bands of the rRNA , then prepared c-DNA and tried to amplify with housekeeping gene primer but i didnt get any amplification. I had stored the rna in -70C for 1 day before cDNA preparation. Is this why i didnt get any amplification. Does this m-rna in the whole rna isolation process degrade so fastly?
Please help me with some suggestions
Posted 23 June 2010 - 04:06 AM
this storage step is no problem. I guess something went wrong during the cDNA synthesis or the PCR.
Posted 23 June 2010 - 05:13 AM