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RT-PCR cyclers


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#1 Nataliya

Nataliya

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Posted 22 June 2010 - 07:10 AM

Hi!

I am looking for the RT-PCR cycler which is compatible with QuantiTect SYBR Green PCR Kits. I can not find any detailed information about Rotor-Gene Q models and the prices. I've been told that there are basic models and multiplex models. The models have from 2 up to 6 channels and the price for them variates accordingly. Does anyone have the good description for the different Rotor-Gene models?

Another question concerns the compatibility of Qiagen Master Mix and primers with ABI RT-PCR cyclers. I failed to use QuantiTect SYBR Green PCR Kit and 7900HT Fast Real-Time PCR System. Does anyone tried to use the kits from Qiagen for ABI machines? Did RT-PCR run successfully?

Nataliya.




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