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cloning into pUAST vector


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#1 cacandice1982

cacandice1982

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Posted 22 June 2010 - 01:31 AM

Hi, I am trying to clone my gene into pUAST vector. I am designing my primers with restriction sites, does anyone have information about the frames of the multiple cloning site. Please help. And is there anyway to check the expression of my gene before injection into drosophila embryos.

Thanks

candice




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