What would be the best way to activate mouse B cells in vitro? How long do they survive in the activated states in vitro culture? Thanks for your advice in advance!
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anonymous
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Posted 31 January 2001 - 10:00 PM
A good way to activate mouse B cells in culture is by stimulating them with LPS at a concentration of 40 micrograms per ml. I have stimulated them up to 72 hours but I have been told that they can last at least a week.
