Posted 21 June 2010 - 09:57 AM
I was wondering if someone could tell me if I can use NaCl (150mM) in a CD experiment? I've heard that it is bad for low wavelengths and that NaF should be substituted where possible. Does anyone know if this is the case or is it just being over-cautious?
Posted 23 June 2010 - 08:44 AM
it says some prefer fluoride instead of chloride salts because they absorb less in the far uv but it doesn't say that chloride salts can't be used.
genius does what it must
i do what i get paid to do
Posted 03 July 2010 - 04:55 AM
See the following informative papers for more info;
Greenfield (2007) using circular dichroism spectra to estimate protein secondary structure
Kelly (2005) how to study proteins by circular dichroism