1 reply to this topic

[wjchxl]

Hi,

I need to do check proliferation of my cell lines in serum free condition.  So I have two choices:

1) trypsinize cells and resuspend cells in 10%FBS medium, plate cells; change medium to serum free the next day;
2) after trypsinizing and resuspending in 10%FBS medium, centrifuge cells, aspirate 10%FBS medium; resuspend cells in serum free medium and plate cells at desired amount.

I would prefer choice 2) since washing and changing medium may cause loss of this specific cell line.  But I don't know if cells can adhere to the plate when plated in serum free medium.  

Any suggestions?  Thanks!

[wjchxl]

By the way, it is LNCaP cells.  Any suggestion?