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MSMS spectral interpretation of permethylated glycan


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#1 mhstin

mhstin

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Posted 20 June 2010 - 11:09 PM

Hi guys,

Any1 from here working on glycobiology especially permethylated glycan? Any of you know how to interpret the MSMS data. Coz manual calculation is totally not accurate... unless with the aid of databeses or software~~~ its hard to predict whether the peak is glycan or not if just based on calculation. Its only suitable for those common high mannose N-glycan (2 glynac.. devide the rest using mass of mannose"). How about other which is extremely big molecules of N-glycan. Besides, O-glycan is even more complex in term of their derivatization. Any1 of you knowing is there any free software to help us analyze the spectra? or even other specific methods/ calculation... solution... bla bla bla~~~ thx a lot~~~




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