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IgG SDS-PAGE - SDS-PAGE and Western Blotting - BioForum

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IgG SDS-PAGE


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#1 Princesslinda

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Posted 19 June 2010 - 04:14 PM

Hi all-

I recently ran a SDS-PAGE gel of a monoclonal antibody (+ 2X SB) and after developing the western, I noticed there were 3 bands on my film. I know I should see a heavy and light chain, but does the third (and largest) band indicate that some of the antibody was not denatured?

Thanks!

#2 Gerard

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Posted 19 June 2010 - 11:57 PM

Maybe this can help.

http://www.aapsj.org...art=aapsj080243
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".




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