2 replies to this topic

[sagagirish]

Hiii
          I have prepared 450 ml of RPMI + 50 ml FBS and added 5 ml of L-glutamine (200 mM)

I have seen in many cases that people use 2mM L-glutamine....Should I prepare and use a 2mM L-glutamine or 2mM is actually the final concentration ?

[bob1]

2mM L-glut is the final concentration in the medium.  You are using 2 mM as well, if you do the calculation.

[sagagirish]

bob1, on Jun 17 2010, 04:36 PM, said:

2mM L-glut is the final concentration in the medium.  You are using 2 mM as well, if you do the calculation.

  


Thanks a lot bob1....i figured it out that my final conc was actaully 2 mM ...thanks anyways