L-glutamine amount and concentration
Posted 17 June 2010 - 08:22 AM
I have prepared 450 ml of RPMI + 50 ml FBS and added 5 ml of L-glutamine (200 mM)
I have seen in many cases that people use 2mM L-glutamine....Should I prepare and use a 2mM L-glutamine or 2mM is actually the final concentration ?
Posted 17 June 2010 - 04:36 PM
Posted 18 June 2010 - 06:56 AM
2mM L-glut is the final concentration in the medium. You are using 2 mM as well, if you do the calculation.
Thanks a lot bob1....i figured it out that my final conc was actaully 2 mM ...thanks anyways