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L-glutamine amount and concentration


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#1 sagagirish

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Posted 17 June 2010 - 08:22 AM

Hiii
I have prepared 450 ml of RPMI + 50 ml FBS and added 5 ml of L-glutamine (200 mM)

I have seen in many cases that people use 2mM L-glutamine....Should I prepare and use a 2mM L-glutamine or 2mM is actually the final concentration ?

#2 bob1

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Posted 17 June 2010 - 04:36 PM

2mM L-glut is the final concentration in the medium. You are using 2 mM as well, if you do the calculation.

#3 sagagirish

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Posted 18 June 2010 - 06:56 AM

2mM L-glut is the final concentration in the medium. You are using 2 mM as well, if you do the calculation.




Thanks a lot bob1....i figured it out that my final conc was actaully 2 mM ...thanks anyways




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