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Running a 15% PAGE


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#1 Fhannan

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Posted 17 June 2010 - 03:22 AM

Hello,
Under what conditions you usually run a 15% PAGE, amp? voltage? time? i am using Bio-Rad gel instrument...

#2 Prep!

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Posted 17 June 2010 - 03:39 AM

Hello,
Under what conditions you usually run a 15% PAGE, amp? voltage? time? i am using Bio-Rad gel instrument...


i guess u are under the wrong section!!!
i tun it at 130V for 1 hour!!
u can even use 100 V till stacking and then increase in separating!!
Best luck!!
Support bacteria - They are the only culture some people have!!!
Cheers!!!

#3 K.B.

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Posted 17 June 2010 - 09:28 PM

I use 80-100V for stacking gel and 100-120V for separating gel. Watch out for overheating at higher voltages.




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