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cell adhesion assay


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#1 SF_HK

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Posted 16 June 2010 - 06:26 PM

Hi,

I'm currently oerforming cell adhesion assays on my cell lines expressing my gene of interest vs cell line transfected with vector only. I'm using a colorimetric method. Howver, I do not understand the rationale behind this assay. If a cell line is tumor suppressive, do we expect it to have more cells adhering, i.e higher abosrbance reading comapred to the control or vice versa?

I thought if a cell line is tumor suppresisve, more cells would adhere, demonstrating reduced metastasis ability?

#2 Inmost sun

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Posted 17 June 2010 - 01:13 AM

Hi,

I'm currently oerforming cell adhesion assays on my cell lines expressing my gene of interest vs cell line transfected with vector only. I'm using a colorimetric method. Howver, I do not understand the rationale behind this assay. If a cell line is tumor suppressive, do we expect it to have more cells adhering, i.e higher abosrbance reading comapred to the control or vice versa?

I thought if a cell line is tumor suppresisve, more cells would adhere, demonstrating reduced metastasis ability?


this may be a feature of carcinoma or adenocarcinoma cells: Attachment gives survival signals which are less needed for carcinoma cells; perform also as a control experiment a poly-HEMA attachment assay




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