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Chromatography Column question


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#1 cancergeek

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Posted 16 June 2010 - 12:32 PM

Hi guys,

Quick question. What purpose does the little white disk (frit) serve for a protein A chromatography column? My co worker says he never uses them and that its unnecessary. Is that true?

Thanks

#2 Inmost sun

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Posted 17 June 2010 - 01:24 AM

Hi guys,

Quick question. What purpose does the little white disk (frit) serve for a protein A chromatography column? My co worker says he never uses them and that its unnecessary. Is that true?

Thanks


it is used to prevent flow through of column matrix material...

#3 K.B.

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Posted 17 June 2010 - 09:44 PM

That would be the bottom frit. Top frit is used to prevent gel disturbance by the flow (sample application, wash/elution/whatever buffer) which may have negative influence on separation.




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