2 replies to this topic

[cancergeek]

Hi guys,

Quick question.  What purpose does the little white disk (frit) serve for a protein A chromatography column? My co worker says he never uses them and that its unnecessary.  Is that true?

Thanks

[Inmost sun]

cancergeek, on Jun 16 2010, 08:32 PM, said:

Hi guys,

Quick question.  What purpose does the little white disk (frit) serve for a protein A chromatography column? My co worker says he never uses them and that its unnecessary.  Is that true?

Thanks

it is used to prevent flow through of column matrix material...

[K.B.]

That would be the bottom frit. Top frit is used to prevent gel disturbance by the flow (sample application, wash/elution/whatever buffer) which may have negative influence on separation.