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Bromophenol blue turns yellow - Molecular Biology - BioForum

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Bromophenol blue turns yellow


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1 reply to this topic

#1 Curtis

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Excellent

Posted 16 June 2010 - 09:12 AM

I've never had this problem before and I have previously made SDS-PAGE buffer without any problem but I don't why today when I was making the buffer it turned yellow. It doesn't matter if I first add SDS or glycerol or whatever, it just turns yellow anyway.

I even used another container of Bromphenol blue but it had the same problem

#2 Gerard

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Neutral

Posted 16 June 2010 - 10:06 AM

It's a problem in your buffer

Bromophenol blue is an acid-base indicator its useful range lies between pH 3.0 and 4.6. It changes from yellow at pH 3.0 to purple at pH 4.6; this reaction is reversible. Bromophenol blue is structurally related to phenolphthalein-a popular indicator.

http://en.wikipedia....romophenol_blue
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".




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