I've never had this problem before and I have previously made SDS-PAGE buffer without any problem but I don't why today when I was making the buffer it turned yellow. It doesn't matter if I first add SDS or glycerol or whatever, it just turns yellow anyway.
I even used another container of Bromphenol blue but it had the same problem
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#1
Curtis
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Posted 16 June 2010 - 09:12 AM
#2
Gerard
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Posted 16 June 2010 - 10:06 AM
It's a problem in your buffer
Bromophenol blue is an acid-base indicator its useful range lies between pH 3.0 and 4.6. It changes from yellow at pH 3.0 to purple at pH 4.6; this reaction is reversible. Bromophenol blue is structurally related to phenolphthalein-a popular indicator.
http://en.wikipedia....romophenol_blue
Bromophenol blue is an acid-base indicator its useful range lies between pH 3.0 and 4.6. It changes from yellow at pH 3.0 to purple at pH 4.6; this reaction is reversible. Bromophenol blue is structurally related to phenolphthalein-a popular indicator.
http://en.wikipedia....romophenol_blue
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