Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Bromophenol blue turns yellow


  • Please log in to reply
1 reply to this topic

#1 Curtis

Curtis

    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,112 posts
59
Excellent

Posted 16 June 2010 - 09:12 AM

I've never had this problem before and I have previously made SDS-PAGE buffer without any problem but I don't why today when I was making the buffer it turned yellow. It doesn't matter if I first add SDS or glycerol or whatever, it just turns yellow anyway.

I even used another container of Bromphenol blue but it had the same problem

#2 Gerard

Gerard

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 124 posts
0
Neutral

Posted 16 June 2010 - 10:06 AM

It's a problem in your buffer

Bromophenol blue is an acid-base indicator its useful range lies between pH 3.0 and 4.6. It changes from yellow at pH 3.0 to purple at pH 4.6; this reaction is reversible. Bromophenol blue is structurally related to phenolphthalein-a popular indicator.

http://en.wikipedia....romophenol_blue
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.