Hallo to everyone!
I am currently starting with a long cloning procedure.
For the beginning I have 2 oligos, 50nucleotides each, 50pmol/ul in ddH20.
I want first to anneal them according the following protocol :
200uM of each oligo
1x oligo buffer
up to 20ul ddH2O water
1): should I dilute them before in TE buffer and from that take 200uM for the reaction?
2): after annealing, should I dilute them more, or directly take from the annealed mixture something like 50nM to use in a ligation reaction with a vector?
Thank you in advance!
oligos for ligation
No replies to this topic