EtBr- post satining protocol
Posted 16 June 2010 - 02:39 AM
Posted 16 June 2010 - 05:43 AM
Posted 16 June 2010 - 06:25 AM
Stain for 10-30 minutes.
You can reuse and store the staining solution up to several months, if you keep it in fridge in the dark.
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Posted 16 June 2010 - 05:45 PM
Usually the solution should be so dilute that you can't see the EtBr colour. If it is reddish, you have added too much.
add EtBr (colour of the buffer should be reddish).