Posted 15 June 2010 - 10:28 AM
I have tried running an agarose gel, which produces two bands. I have thought about cutting out the larger band, but this is complicated by the fact that single stranded DNA does not migrate in a predictable fashion on a gel.
Any advice would be appreciated.
Posted 16 June 2010 - 03:56 AM
Posted 16 June 2010 - 04:23 PM
However, if your product is <100bp or there is only one product in your reaction then using an combination of Exonuclease I and Shrimp Alkaline Phosphatase can be used to remove oligos. ExoSAP-IT is product by GE that does this but there are plenty of companies that have an equivalent product.