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Moving S2 cells from one plate to another


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#1 mcb56

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Posted 15 June 2010 - 06:16 AM

Hello, I have seeded Drosophilia S2 cells from a main 75 cm2 flask into 6 well plates at a cell concentration of 5x10^6 cells/ml with 2ml/well (1x10^7 cells/well). I let the cells sit in the wells for 30 minutes before adding a drug to them. Is 30 minutes a sufficient amount of time for the cells to get acclimated to their new surroundings before adding a drug or should I have let them sit longer? The drug is a protein kinase B inhibitor and it doesn't hurt the cells too much.

Thank you

#2 telamon

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Posted 25 June 2010 - 12:00 PM

Hello, I have seeded Drosophilia S2 cells from a main 75 cm2 flask into 6 well plates at a cell concentration of 5x10^6 cells/ml with 2ml/well (1x10^7 cells/well). I let the cells sit in the wells for 30 minutes before adding a drug to them. Is 30 minutes a sufficient amount of time for the cells to get acclimated to their new surroundings before adding a drug or should I have let them sit longer? The drug is a protein kinase B inhibitor and it doesn't hurt the cells too much.

Thank you


are they suspension? if you are only changing the physical environment (wells, temperature from leaving incubator...) the day of the experiment, i would not wait much time at all after i changed wells... simply to get them back in the incubator faster. i suppose you could change the well then re incubate them for 30 minutes before adding the drug, but honestly if it were me i would not take my plates in and out of the incubator/hood and removing the plate cover often, just increases chances of infection. I would have all my media/plates prepared, remove from incubator and aliquot into the wells that have the conditions (drug or controls) and place back in incubator if that makes sense. just a noob here tho mayb others give u more advice with this bump




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