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Nucleofection of ES cells, medium instead of nucleofector solution?

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#1 autophagator



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Posted 15 June 2010 - 05:55 AM

In our lab we have Human Cancer Stem Cells derived of pacients and I need to introduce a forgein DNA into them, but all methods fail to tranfect primary cells. So, I will use the Nucleofector Technology, but I have not the expensive nucleofector solution...
I would like to know how I can replace the expensive Nuclefector solution to electroporate my ES cells. Can I use cell culture medium of Cancer Stem Cells?

thanks in advance.

#2 vitalgene



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Posted 12 January 2011 - 04:20 AM

cultre medium doent work with nucleofectors, try some transfecting reagents (without use of neuclefectortm) like nanojuice,. lipofectamin, they reduced the prices for it and also comes a free sample kits for testing
good luck

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