Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Nucleofection of ES cells, medium instead of nucleofector solution?


  • Please log in to reply
1 reply to this topic

#1 autophagator

autophagator

    member

  • Active Members
  • Pip
  • 27 posts
0
Neutral

Posted 15 June 2010 - 05:55 AM

Hi.
In our lab we have Human Cancer Stem Cells derived of pacients and I need to introduce a forgein DNA into them, but all methods fail to tranfect primary cells. So, I will use the Nucleofector Technology, but I have not the expensive nucleofector solution...
I would like to know how I can replace the expensive Nuclefector solution to electroporate my ES cells. Can I use cell culture medium of Cancer Stem Cells?


thanks in advance.

#2 vitalgene

vitalgene

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 53 posts
0
Neutral

Posted 12 January 2011 - 04:20 AM

cultre medium doent work with nucleofectors, try some transfecting reagents (without use of neuclefectortm) like nanojuice,. lipofectamin, they reduced the prices for it and also comes a free sample kits for testing
good luck




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.