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[amacdonald]

Hi. Does anyone know the acceptable level of endotoxin in endotoxin units (EU) per mL?  I've just performed Sigma's E-Toxate LAL assay and the standards are expressed in EU/mL.  All of the culture media specifications I've seen express endotoxin levels in ng/mL.

[gaynour]

The acceptable level of endotoxin depends on which cells you are looking at, and how they respond to endotoxin.
The E-Toxate endotoxin standard is standardized against the US Reference Standard Endotoxin.  The ratio RSE/E-Toxate standard gives the potency: around 10EU/ng.