3 replies to this topic

[wenqi]

Hi ,
    I would like to use sodium azide as a preservative for bovine serum albumin. the concentration I am using for BSA is 2mg/ml. so what concentration of sodium azide should I use such that BSA gets preserved?

thanks

[parado]

wenqi, on Jun 15 2010, 11:02 AM, said:

Hi ,
    I would like to use sodium azide as a preservative for bovine serum albumin. the concentration I am using for BSA is 2mg/ml. so what concentration of sodium azide should I use such that BSA gets preserved?

thanks

the normally used concentration for Na3N is 0.2%, but i always keep my BSA at -20C  or just prepare it each time i need.

Edited by parado, 14 June 2010 - 07:38 PM.

[wenqi]

Hi parado , thanks for that info, would you have any paper or journal where I can reference that concentration used? Thanks

Edited by wenqi, 21 June 2010 - 08:11 PM.

[sgt4boston]

0.2% is very common. You can store at 4C until it becomes cloudy.