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preserving bovine serum albumin with sodium azide


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3 replies to this topic

#1 wenqi

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Posted 14 June 2010 - 07:02 PM

Hi ,
I would like to use sodium azide as a preservative for bovine serum albumin. the concentration I am using for BSA is 2mg/ml. so what concentration of sodium azide should I use such that BSA gets preserved?

thanks

#2 parado

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Posted 14 June 2010 - 07:37 PM

Hi ,
I would like to use sodium azide as a preservative for bovine serum albumin. the concentration I am using for BSA is 2mg/ml. so what concentration of sodium azide should I use such that BSA gets preserved?

thanks



the normally used concentration for Na3N is 0.2%, but i always keep my BSA at -20C or just prepare it each time i need.

Edited by parado, 14 June 2010 - 07:38 PM.


#3 wenqi

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Posted 21 June 2010 - 08:10 PM

Hi parado , thanks for that info, would you have any paper or journal where I can reference that concentration used? Thanks

Edited by wenqi, 21 June 2010 - 08:11 PM.


#4 sgt4boston

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Posted 22 June 2010 - 02:56 AM

0.2% is very common. You can store at 4C until it becomes cloudy.




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