3 replies to this topic

[NinerFan49]

Hi everyone, I'm new here. I was careless and left my whole sample of plasmid DNA out for a week. I want do a pcr; will it be ok to use the plasmd?

[Trof]

If by "out" you mean on room temperature, then I can tell you I once forgot plasmids whole month in the corner of the bench. They were send dryed on the whatman paper over the ocean and successfuly transfected into bacteria.

I think plasmids survive a lots of things if they are in the right buffer.

[NinerFan49]

Trof, on Jun 15 2010, 05:35 AM, said:

If by "out" you mean on room temperature, then I can tell you I once forgot plasmids whole month in the corner of the bench. They were send dryed on the whatman paper over the ocean and successfuly transfected into bacteria.

I think plasmids survive a lots of things if they are in the right buffer.

Thanks for your input.

Yes, I mean at room temperature. That's a relief, they are in tris buffer, which should be good then.

[HomeBrew]

DNA's natural state is quite a bit warmer than room temperature (in mammals, anyway). Also, DNA survives quite well after repeated and prolonged exposure to high temperatures in a PCR reaction. It's all about how clean your DNA is (no nucleases) and what it's stored in (some buffer rather than water).