Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

What is the solution when tween80 or DMSO is not enough to dissolve plant extrac


  • Please log in to reply
2 replies to this topic

#1 Ahmed Alrifai

Ahmed Alrifai

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 14 June 2010 - 09:34 AM

Hi,
I'm stuck on this thought, can you please help me!?

I'm study the effect of some plant extracts (by organic solvent: hexan. chloroform, EA and methanol) on nematode larvae.... I have to use just 3% of DMSO or Tween80 to dissolve 5 grams of extract to prepare 100ml of 50mg/ml extract as stock by dissolving 5g of extract in 3ml of DMSO or Tween80 and add 97ml distilled water and then I'll use this concentrations 50, 25, 12.5, 6.3 and 3.2 but the problem is 3ml is not enough to dissolve 5g of extract and If I add more DMSO OR Tween it'll be toxic for the larvae?

So, how to solve this problem

Ahmed

#2 parado

parado

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 14 June 2010 - 11:14 PM

Hi,
I'm stuck on this thought, can you please help me!?

I'm study the effect of some plant extracts (by organic solvent: hexan. chloroform, EA and methanol) on nematode larvae.... I have to use just 3% of DMSO or Tween80 to dissolve 5 grams of extract to prepare 100ml of 50mg/ml extract as stock by dissolving 5g of extract in 3ml of DMSO or Tween80 and add 97ml distilled water and then I'll use this concentrations 50, 25, 12.5, 6.3 and 3.2 but the problem is 3ml is not enough to dissolve 5g of extract and If I add more DMSO OR Tween it'll be toxic for the larvae?

So, how to solve this problem

Ahmed


i always use the organic solvent to prepare the stock solution, then add them to water, disregard its solubility. ...

#3 Ahmed Alrifai

Ahmed Alrifai

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 17 June 2010 - 12:38 AM

I tried one time and I got two layers the first is extract with organic solvent and the second is the water in the bottom and the larvae were moving inside the water so these was no effecting of extract on the larvae

i always use the organic solvent to prepare the stock solution, then add them to water, disregard its solubility. ...






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.