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Agarose Gel pretreatment before phenol/chloroform DNA extraction


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#1 mahsa

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Posted 14 June 2010 - 03:42 AM

hey there
I have got this DNA fragment which is of 81 bp length and I have to extract it according to phenol/chloroform protocol because I don't have a gel extraction kit with appropriate cut-off size in my hand. I wonder if any one can comment me on the very first step of getting rid of the gel, like should i melt it( which is not a very clever way obviously 'cos it is a 3% agarose gel) or any other possible and reasonable way. And please forget about filter papers 'cos I don't have access to those too.

tnx a alot
any comments or ideas are appreciated
waiting here in eager





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