- After addition of Lipofectamine to Plasmid, do we need to vortex the mixture vigorously? or just pipetting up and down would be enough?
- Do we need to rotate the plate once in a while after transfection?
- Do we need to remove lipofectamine after 4-6 hours post-transfection and add fresh media?
- Is 16 hours post-transfection enough for cell lysis to detect GFP expression by western blot? what is the recommended time?
Edited by Curtis, 14 June 2010 - 01:04 AM.