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[drdineshghadi]

HELLOW EVERYONE

HERE ITS DR DANZ....
I M WORKING ON EXPRESSION STUDY OF TLR GENE IN MASTITIS USING REAL TIME PCR
I AM WORKING ON AB7500 FAST REAL TIME SYSTEM AND GETIING GOOD EXPRESSION-
GOOD AMPLIFICATION PLOTS AND MELT CURVE.

BUT THE PROBLEM IS THAT ,THE PCR  PRODUCTS WHICH I GET AFTER RUNNING REAL TIME PCR ARE  NOT PROPERLY  VISUALISED ON AGAROSE GEL (2%)

THE SAME PRODUCT WHEN DONE BY NORMAL PCR SHOWS BANDC OF APROPRIATE SIZE(200 BP)

I M USING QUIAGEN QUANTIFAST SYBR GREEN MASTER MIX

ANY SUGGESSION IS APRECIABLE

THANKS

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