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well washer


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6 replies to this topic

#1 Faisl

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Posted 12 June 2010 - 01:05 AM

Hi
I have been trying to do competitive ELISA however, I haven't well washer.So my question is; can I run my experiment with washing manually?
Many thanks for any suggestion.

#2 K.B.

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Posted 12 June 2010 - 05:23 AM

In my lab we don't have plate washer and we do all kind of ELISA including competitive. We wash wells manually three times using multi-channel pipette.

#3 Gerard

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Posted 12 June 2010 - 06:14 AM

Take care that no wash solution stays put in the plate otherwise you get problems.
Hit the platea few times on a papertowel on the bench.
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".

#4 Faisl

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Posted 13 June 2010 - 09:05 AM

Take care that no wash solution stays put in the plate otherwise you get problems.
Hit the platea few times on a papertowel on the bench.



Best thanks for all your replies

#5 sgt4boston

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Posted 15 June 2010 - 12:21 PM

I agree, blot vigorously several times between washes using fresh / clean paper towel between blottings.

Additionally some protocols call for leaving the wash solution in wells for 15sec between blottings.

Multichannel pipettor is not absolutely necessary...a squeeze bottle can also be used...as long as the number of washes is sufficient.

between after last wash step and before reagent addition (non-wash) allow the plate to remain inverted on clean paper towel to completely drain.

good luck.

#6 K.B.

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Posted 16 June 2010 - 06:16 AM

I tried using squeeze bottle - maybe I was doing it wrong but it was quite messy and you use more buffer for washing.

#7 sgt4boston

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Posted 16 June 2010 - 10:58 AM

Yes squeeze bottle is messy but quicker.

there are automated washers, mainfolds that you can attach to bottle top dispenser, multichannel pipettor and lastly squeeze bottle. Just depends upon what you have available or what you are willing to spend.




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