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Methods of tranformation

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#1 fishy



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Posted 10 June 2010 - 08:17 PM


I have been doing transformation by the electroporation method for my cloning and I use a ligated plasmid for transformation. however i have never been successful in the cloning. Apart from trouble shooting other areas, I was wondering if the method of transformation could have a role in this. could chemical method of transformation yield the desired result when i am trying to clone with a circularised plasmid as compared to a linear plasmid? Is there a difference between electroporation and the chemical method of transformation apart from the yield?

Please let me know...

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