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Methanol vs Isopropanol in fixing solution


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#1 polaris

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Posted 10 June 2010 - 08:46 AM

General question: What is the difference between using methanol/acetic acid versus isopropanol/acetic acid when fixing proteins in gels?

#2 mdfenko

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Posted 23 June 2010 - 10:36 AM

functionally, no difference.

concentration of alcohol necessary is different (i think you need 10% isopropanol as opposed to 30-50% methanol).
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