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GFP and Luciferase Cell Line


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#1 aks

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Posted 10 June 2010 - 07:17 AM

Hi,
I am looking for a cell line that has both GFP and Luciferase. I found cell lines in ATCC that are either GFP positive or Luciferase positive. Would be very pleased if you could help me find the cell line that i am looking for. Thanks in advance!

Aks

#2 WYF

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Posted 10 June 2010 - 07:48 AM

Can you not get the cell lines expressing either GFP or luciferase, then transfect the other?

#3 rkay447

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Posted 10 June 2010 - 08:33 AM

There is an article entitled "The Renilla Luciferase-Modified GFP Fusion Protein is Functional in Transformed Cells". Authors are Yubao Wang, Gefu Wang, Dennis J. O'Kane and Aladar A. Szalay. It's in a book/journal BioHydrogen and was published in August of 2007. I don't have access to this journal but the abstract describes mammalian cell lines with a fusion protein of GFP and luciferase that are functional by FRET analysis. You may want to write the corresponding author to get more information.




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